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We have many excellent staff members good at marketing, QC, and dealing with kinds of troublesome problem in the production process for Protein Purification Pdf,Chromatography Separation and Purification,UniMab 50L Protein A. we've constantly been around the forefront of clean technology merchandise innovation. We are a green partner you can rely on. Call us today for more data!
Nanomicrotech has the world's leading technology in chromatography media, and the company also has rich experiences in the development of purification processes.
Characteristics and Advantages
Highly uniform particles
Uni®Mab affinity chromatography medium has highly uniform particle size distribution and precisely controlled porestructure, as shown in Fig. 1.
Figure 1. SEM diagram of Uni®Mab and a leading brand of protein A medium
High flow rate
Uni®Mab affinity chromatography medium was made from polymethyl methacrylate (PMMA) microspheres (as matrix); and it would withstand the pressure of 0.5 MPa. When compared to an affinity medium with convention alagarose matrix of the chromatography media, it would make the separation process much faster, thus reducing the time purification, for hundreds of liters of large scale chromatography preparative column.(Experimental:Column:4.6 mm × 50 mm Mobile phase:50 mM PBS,0.5 M NaCl)
Figure 2. Comparison of Uni®Mab and a conventional agarose type protein A medium, their pressure vs. linear flow rate.
Excellent loading capacity
At a high flow rate, Uni®Mab showed a significantly better dynamic binding capacity (DBC) than that of the conventional agarose type protein A medium. The same observation was also valid, even when the residence time was increase to 4 - 6 minute.(Experimental:Column:7 mm × 25 mm Sample:Human IgG,2 mg/mL Equilibration buffer:20 mM PBS,pH 7.0 ,0.15 M NaCl Elution:0.1 M Glycine ,pH 3.0)
Figure 3. Comparison of Uni®Mab with a leading brand of Protein A medium, for their dynamic binding capacity
Outstanding pH stability
Uni®Mab affinity chromatography medium could be applied in a pH range from 3 - 12, and it could be cleaned with 0.1 - 0.5 M NaOH. In a life-cycle test, Uni®Mab medium remained greater than 90% of the original dynamic binding capacity (DBC), after 100 CIP cycles with 0.5 M NaOH as CIP reagent. (CIP procedure:Medium:Uni®Mab Column:7 mm × 25 mm Sample:recombinant antibody(chimera) DBC calculation:10% breakthrough Equilibration buffer:20 mM PBS,150 mM NaCl, pH 7.2,3 CV(column volume) Elution:20 mM Citrate,pH 3.0 CIP:0.5 M NaOH,15 CV Re-equilibration:10 CV)
Figure 4. Uni®Mab pH stability in life cycle tests
Uni®Mab was secessfully applied in purification of hIgG from human serum. (Experimental:Column:7 mm× 25 mm Sample:human serum,5 mL Equilibration Buffer:20mM PBS,pH 7.0
0.15 M NaCl Elution:0.1 M Glycine ,pH 3.0 Residence time:4 min )
Figure 5.Uni®Mab used in hIgG purification from human serum
Pre-packed column cartridge
We are proud to be the sole producer (and provider) of monodisperse, super pure silica gel chromatographic media in the world; we also provide monodisperse polymer chromatographic media (these products will match the performance of similar GE products).
"We'll make each individual effort to become exceptional and ideal, and speed up our steps for standing inside the rank of worldwide top-grade and high-tech enterprises for Protein A Affinity Chromatography Medium. The product will supply to all over the world, such as: Belize,Virgin Islands (British),Qatar. Now we professionally supplies customers with our main goods And our business is not only the "buy" and "sell" but also focus on more. We target to be your loyal supplier and long-term cooperator in China. Now We hope to be the friends with you.
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