Company Details

  • Suzhou Nanomicro Technology Co., Ltd.

  •  [Jiangsu,China]
  • Business Type:Manufacturer
  • Main Mark: Americas , Asia , Europe , Worldwide , Africa , Caribbean , East Europe , Middle East , North Europe , Oceania , Other Markets , West Europe
  • Exporter:31% - 40%
  • Certs:ISO9001, MSDS
  • Description:Column Chromatography Applications,Principle of Column Chromatography,Uni HR Phenyl-30L
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Suzhou Nanomicro Technology Co., Ltd.

Column Chromatography Applications,Principle of Column Chromatography,Uni HR Phenyl-30L

HIC Chromatography Media Uni-HR Phenyl-30L

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    Payment Type: L/C,T/T
    Incoterm: FOB,CFR,CIF

Basic Info

Model No.: Uni-HR Phenyl-30L

Pore Size: 1000

Functional Group: Phenyl

Additional Info

Transportation: Ocean,Air

Product Description

Nanomicro Tech offers a series of hydrophobic interaction chromatography (HIC) media for high efficiency separation and purification of proteins and peptides. HIC is used to separate proteins on the basis of relative hydrophobicity. While in reversed phase column chromatography (RPC), desorption is often promoted by the addition of an increasing amount of organic solvent. Therefore gentle binding and elution condition of HIC allows a better retaining of the biologic activity of the target protein than RPC in which organic solvents are applied. The strength of the hydrophobic interaction is influenced strongly by the nature of the salt components in the mobile phase. The salt concentration needed depends on the protein hydrophobicity and solubility. HIC is particularly attractive for Protein Purification after ion exchange and affinity chromatography, where the sample with high salt concentration can often be applied directly to HIC. By combining HIC with ion exchange or affinity chromatography, the purification of biomolecules can be completed efficiently.

Different Types Of Chromatography

Features and Benefits

Features

Benefits

Highly uniform particle size

Easy column packing, high resolution

Optimised pore structure and surface bonding

High loading capacity and high selectivity

Superior chemical and pH stability

Easy cleaning, long lifetime

No filter impurities

Eliminate non-specific bindings

High mechanical strength

Low back pressure, High linear velocity

Large-scale manufacturing capability

Consistent product quality and supply

Neutral hydrophilic modification

high yield

Highly uniform particle size

Uni®HIC are designed to deliver high resolution and high recovery for both lab and large-scale purification. Highly uniform particle sizes of 15, 30, or 80 μm with a narrow particle size distribution are available.

Superior chemical and pH stability

Column Chromatography Definition


Comparison of column efficiency after treatment with acid and base

NanoHR Phenyl-15L

§ Uni®HR Butyl-30L Phenyl-15L

High Binding Capacity

HIC Chromatography Media


Column: 4.6 mm x 50mm

Sample: Lysozyme (1mg/mL) in 1.8mol/L (NH4)2SO4 + 100mM sodium phosphate (pH 7.0)

Buffer: 100mM sodium phosphate (pH 7.0)

Flow Rate: 0.5mL/min (180cm/h)

Detector:AKTA Purifier (UV@280nm)

Dynamic binding capacity (DBC) of Nano-Micro Tech and competitor HIC resins

Products List


Series

Product Name

Diameter

Pore Szie

Functional Group

Features/

Benefits

Application

μm

Å

UniÒHR

UniÒHR Phenyl-80L

80

1000

Phenyl

Eliminate non-specific bindings

Ideal for high resolution seperation of proteins and peptides

UniÒHR Phenyl-30L

32

1000

Phenyl

UniÒHR Phenyl-60S

60

500

Phenyl

UniÒHR Phenyl-30S

35

500

Phenyl

UniÒHR Butyl-80L

80

1000

Butyl

UniÒHR Butyl-30L

32

1000

Butyl

UniÒHR Butyl-60S

60

500

Butyl

UniÒHR Butyl-30S

35

500

Butyl

NanoHR

NanoHR Phenyl-15L

15

1000

Phenyl

High resolution/ High mechanical strength /High chemical stability

Ideal for fast and high polishing and analysis of proteins and peptides

NanoHR Butyl-15L

15

1000

Butyl


Looking for ideal Column Chromatography Applications Manufacturer & supplier ? We have a wide selection at great prices to help you get creative. All the Principle of Column Chromatography are quality guaranteed. We are China Origin Factory of Uni HR Phenyl-30L. If you have any question, please feel free to contact us.

Product Categories : Chromatography Media - Monodisperse > Hydrophobic Interaction Chromatography Media

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  • HIC Chromatography Media Uni-HR Phenyl-30L
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