Company Details

  • Suzhou Nanomicro Technology Co., Ltd.

  •  [Jiangsu,China]
  • Business Type:Manufacturer
  • Main Mark: Americas , Asia , Europe , Worldwide , Africa , Caribbean , East Europe , Middle East , North Europe , Oceania , Other Markets , West Europe
  • Exporter:31% - 40%
  • Certs:ISO9001, MSDS
  • Description:Affinity Chromatography Procedure,Chromatography Separation and Purification,UniMab 50L Recomibiant
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Suzhou Nanomicro Technology Co., Ltd.

Affinity Chromatography Procedure,Chromatography Separation and Purification,UniMab 50L Recomibiant

Home > Products > Chromatography Media - Monodisperse > Protein A Chromatography Media > Protein A Affinity Chromatography Medium UniMab 50L Recombinant

Protein A Affinity Chromatography Medium UniMab 50L Recombinant

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    Payment Type: L/C,T/T
    Incoterm: FOB,CFR,CIF

Basic Info

Model No.: UniMab 50L Recombinant

Pore Size: 1000

Functional Group: Recombinant

Additional Info

Transportation: Ocean,Air

Product Description

Protein A Affinity Chromatography Medium

Uni®Mab is a high performance Protein A affinity chromatography medium, introduced by Nanomicrotechwithits patented technology developed in-house. This medium is based on the monodisperse porous poly (methyl methacrylate) (PMMA) microspheres as the matrix, and a genetically modified protein A as the ligand, which ensures its high affinity binding of monoclonal antibodies and Fc fragment containing recombinant proteins (macromolecules). Uni®Mab has come with high mechanical strength and good pH stability. Even at a high flow rate, it still maintained a high dynamic binding capacity, meeting the demands from laboratory preparationto industrial production.

Affinity Chromatography Procedure

Characteristics and Advantages

Chromatography Separation and Purification

Highly uniform particles
Uni®Mab affinity chromatography medium has highly uniform particle size distribution and precisely controlled porestructure, as shown in Fig. 1.

Protein A Sepharose

Figure 1. SEM diagram of Uni®Mab and a leading brand of protein A medium

High flow rate
Uni®Mab affinity chromatography medium was made from polymethyl methacrylate (PMMA) microspheres (as matrix); and it would withstand the pressure of 0.5 MPa. When compared to an affinity medium with convention alagarose matrix of the Chromatography Media, it would make the separation process much faster, thus reducing the time purification, for hundreds of liters of large scale chromatography preparative column.(Experimental:Column:4.6 mm × 50 mm Mobile phase:50 mM PBS,0.5 M NaCl)

Protein Purification Methods
Figure 2. Comparison of Uni®Mab and a conventional agarose type protein A medium, their pressure vs. linear flow rate.

Excellent loading capacity
At a high flow rate, Uni®Mab showed a significantly better dynamic binding capacity (DBC) than that of the conventional agarose type protein A medium. The same observation was also valid, even when the residence time was increase to 4 - 6 minute.(Experimental:Column:7 mm × 25 mm Sample:Human IgG,2 mg/mL Equilibration buffer:20 mM PBS,pH 7.0 ,0.15 M NaCl Elution:0.1 M Glycine ,pH 3.0)

Protein A Chromatography
Figure 3. Comparison of Uni®Mab with a leading brand of Protein A medium, for their dynamic binding capacity

Outstanding pH stability
Uni®Mab affinity chromatography medium could be applied in a pH range from 3 - 12, and it could be cleaned with 0.1 - 0.5 M NaOH. In a life-cycle test, Uni®Mab medium remained greater than 90% of the original dynamic binding capacity (DBC), after 100 CIP cycles with 0.5 M NaOH as CIP reagent. (CIP procedure:Medium:Uni®Mab Column:7 mm × 25 mm Sample:recombinant antibody(chimera) DBC calculation:10% breakthrough Equilibration buffer:20 mM PBS,150 mM NaCl, pH 7.2,3 CV(column volume) Elution:20 mM Citrate,pH 3.0 CIP:0.5 M NaOH,15 CV Re-equilibration:10 CV)

Protein Purification Techniques
Figure 4. Uni®Mab pH stability in life cycle tests

Application
Uni®Mab was secessfully applied in purification of hIgG from human serum. (Experimental:Column:7 mm× 25 mm Sample:human serum,5 mL Equilibration Buffer:20mM PBS,pH 7.0

0.15 M NaCl Elution:0.1 M Glycine ,pH 3.0 Residence time:4 min )

Protein A Affinity Chromatography
Figure 5.Uni®Mab used in hIgG purification from human serum

Technical information

Purification Of Proteins

Pre-packed column cartridge

Protein A Purification

About Nanomicrotech
We are proud to be the sole producer (and provider) of monodisperse, super pure silica gel chromatographic media in the world; we also provide monodisperse polymer chromatographic media (these products will match the performance of similar GE products). Nanomicrotech Co. is one of a few companies in the world, with a capacity of commercial-scale production for both polymer chromatographic media and super pure silica gel media. Currently, the company currently offers most kinds of purification media, covering the positive phase, reverse phase, ion exchange, hydrophobic and affinity chromatography.


Looking for ideal Affinity Chromatography Procedure Manufacturer & supplier ? We have a wide selection at great prices to help you get creative. All the Chromatography Separation and Purification are quality guaranteed. We are China Origin Factory of UniMab 50L Recomibiant. If you have any question, please feel free to contact us.

Product Categories : Chromatography Media - Monodisperse > Protein A Chromatography Media

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