Company Details

  • Suzhou Nanomicro Technology Co., Ltd.

  •  [Jiangsu,China]
  • Business Type:Manufacturer
  • Main Mark: Americas , Asia , Europe , Worldwide , Africa , Caribbean , East Europe , Middle East , North Europe , Oceania , Other Markets , West Europe
  • Exporter:31% - 40%
  • Certs:ISO9001, MSDS
  • Description:Protein Purification Pdf,Chromatography Separation and Purification,UniMab 50L Protein A
Inquiry Basket ( 0 )

Suzhou Nanomicro Technology Co., Ltd.

Protein Purification Pdf,Chromatography Separation and Purification,UniMab 50L Protein A

Home > Products > Chromatography Media - Monodisperse > Protein A Chromatography Media > Protein A Affinity Chromatography Medium

Protein A Affinity Chromatography Medium

Share to:  
    Payment Type: L/C,T/T
    Incoterm: FOB,CFR,CIF

Basic Info

Model No.: UniMab 50L Protein A

Pore Size: 1000

Functional Group: Protein A

Additional Info

Transportation: Ocean,Air

Product Description

Nanomicrotech has the world's leading technology in Chromatography Media, and the company also has rich experiences in the development of purification processes.

Protein A Column

Characteristics and Advantages

Protein A Resin

Highly uniform particles
Uni®Mab affinity chromatography medium has highly uniform particle size distribution and precisely controlled porestructure, as shown in Fig. 1.

Affinity Purification

Figure 1. SEM diagram of Uni®Mab and a leading brand of protein A medium

High flow rate

Uni®Mab affinity chromatography medium was made from polymethyl methacrylate (PMMA) microspheres (as matrix); and it would withstand the pressure of 0.5 MPa. When compared to an affinity medium with convention alagarose matrix of the chromatography media, it would make the separation process much faster, thus reducing the time purification, for hundreds of liters of large scale chromatography preparative column.(Experimental:Column:4.6 mm × 50 mm Mobile phase:50 mM PBS,0.5 M NaCl)


Protein A Separation
Figure 2. Comparison of Uni®Mab and a conventional agarose type protein A medium, their pressure vs. linear flow rate.

Excellent loading capacity
At a high flow rate, Uni®Mab showed a significantly better dynamic binding capacity (DBC) than that of the conventional agarose type protein A medium. The same observation was also valid, even when the residence time was increase to 4 - 6 minute.(Experimental:Column:7 mm × 25 mm Sample:Human IgG,2 mg/mL Equilibration buffer:20 mM PBS,pH 7.0 ,0.15 M NaCl Elution:0.1 M Glycine ,pH 3.0)

Gst Protein Purification
Figure 3. Comparison of Uni®Mab with a leading brand of Protein A medium, for their dynamic binding capacity

Outstanding pH stability
Uni®Mab affinity chromatography medium could be applied in a pH range from 3 - 12, and it could be cleaned with 0.1 - 0.5 M NaOH. In a life-cycle test, Uni®Mab medium remained greater than 90% of the original dynamic binding capacity (DBC), after 100 CIP cycles with 0.5 M NaOH as CIP reagent. (CIP procedure:Medium:Uni®Mab Column:7 mm × 25 mm Sample:recombinant antibody(chimera) DBC calculation:10% breakthrough Equilibration buffer:20 mM PBS,150 mM NaCl, pH 7.2,3 CV(column volume) Elution:20 mM Citrate,pH 3.0 CIP:0.5 M NaOH,15 CV Re-equilibration:10 CV)

7
Figure 4. Uni®Mab pH stability in life cycle tests

Application
Uni®Mab was secessfully applied in purification of hIgG from human serum. (Experimental:Column:7 mm× 25 mm Sample:human serum,5 mL Equilibration Buffer:20mM PBS,pH 7.0

0.15 M NaCl Elution:0.1 M Glycine ,pH 3.0 Residence time:4 min )

Affinity Chromatography Protein Purification
Figure 5.Uni®Mab used in hIgG purification from human serum

Technical information

Protein Purification Column

Pre-packed column cartridge

Protein A Chromatography

About Nanomicrotech
We are proud to be the sole producer (and provider) of monodisperse, super pure silica gel chromatographic media in the world; we also provide monodisperse polymer chromatographic media (these products will match the performance of similar GE products).


Looking for ideal Protein Purification Pdf Manufacturer & supplier ? We have a wide selection at great prices to help you get creative. All the Chromatography Separation and Purification are quality guaranteed. We are China Origin Factory of UniMab 50L Protein A. If you have any question, please feel free to contact us.

Product Categories : Chromatography Media - Monodisperse > Protein A Chromatography Media

Product Images
  • Protein A Affinity Chromatography Medium
Email to this supplier
  • *Subject:
  • *Messages:
    Your message must be between 20-8000 characters
Communicate with Supplier?Supplier
Yuhua Fu Ms. Yuhua Fu
What can I do for you?
Contact Supplier